8RPX
NhoI restriction endonuclease in complex with quadruply methylated DNA target
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 114.635, 167.059, 56.514 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.810 - 1.810 |
R-factor | 0.2065 |
Rwork | 0.205 |
R-free | 0.24270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.101 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.810 | 1.875 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.071 | 1.125 |
Rmeas | 0.075 | 1.170 |
Rpim | 0.021 | 0.317 |
Number of reflections | 99666 | 9866 |
<I/σ(I)> | 18.05 | 2.03 |
Completeness [%] | 99.8 | 99.73 |
Redundancy | 13.2 | 13.3 |
CC(1/2) | 0.998 | 0.911 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M Tris-BICINE, pH 8.5, 20 % v/v ethylene glycol, 10 % w/v PEG 8000, 20 mM 1,6-hexanediol, 20 mM 1-butanol, 20 mM 1,2-propanediol, 20 mM 2-propanol, 20 mM 1,4-butanediol, 20 mM 1,3-propanediol |