8RPR
Crystal Structure of SgvM methyltransferase in complex with alpha-ketoleucine and Zn2+ ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-04 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.2802 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.031, 67.031, 182.745 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.140 |
| R-factor | 0.1965 |
| Rwork | 0.195 |
| R-free | 0.23160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.647 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 2.250 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.080 | 1.380 |
| Number of reflections | 43575 | 23727 |
| <I/σ(I)> | 21.9 | |
| Completeness [%] | 99.1 | |
| Redundancy | 24.2 | |
| CC(1/2) | 0.990 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM Tris/HCl pH 8.5, 250 mM Li2SO4, and 23% PEG 3350 |
