8RPF
Crystal structure of an alcohol-oxidase from Sphingobacterium daejeonense
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.985, 76.780, 88.222 |
| Unit cell angles | 90.00, 98.35, 90.00 |
Refinement procedure
| Resolution | 64.340 - 2.000 |
| R-factor | 0.16564 |
| Rwork | 0.164 |
| R-free | 0.20791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.565 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.290 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | 0.150 |
| Number of reflections | 71851 | 10383 |
| <I/σ(I)> | 12.4 | 5.3 |
| Completeness [%] | 98.2 | 97.5 |
| Redundancy | 2.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | 10%w/vPEG20000, 20%v/vPEGMME550 0.02Mof each alcohol 0.1MMES/imidazole pH6.5 |
