8RPE
Crystal structure of the SMA:VH1 Complex at 1.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-29 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.813, 53.544, 99.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.149 - 1.200 |
| Rwork | 0.112 |
| R-free | 0.14370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.976 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.540 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.036 | 0.167 |
| Rpim | 0.022 | 0.127 |
| Number of reflections | 63942 | 9208 |
| <I/σ(I)> | 22.1 | 4.8 |
| Completeness [%] | 97.8 | 79.7 |
| Redundancy | 6.2 | |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Bis-Tris buffer at pH 5.5 and 20% PEG3350 |
