8RPD
Crystal structure of the SMA homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-30 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 56.816, 56.816, 59.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.424 - 2.150 |
| Rwork | 0.190 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.725 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 2.190 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.107 | 0.835 |
| Rpim | 0.094 | 0.768 |
| Number of reflections | 6216 | 411 |
| <I/σ(I)> | 5 | 1 |
| Completeness [%] | 98.5 | 82.1 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.994 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M HEPES buffer pH 7.0, 1M Succinic acid and 1% w/v PEG2000 MME |
