8RPB
Structure of S79 Fab in complex with IgV domain of human PD-L1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 185.852, 185.852, 110.237 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.240 - 2.794 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (8-JUN-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.240 | 2.940 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.205 | 3.089 |
| Rpim | 0.043 | 0.637 |
| Number of reflections | 28276 | 3987 |
| <I/σ(I)> | 11.8 | 1.3 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 24 | 23.9 |
| CC(1/2) | 0.995 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1M Tris pH 8.0, 1.6M Li2SO4 |
