8RP7
Glutaminase subunit of aminodeoxychorismate synthase from Escherichia coli
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976200 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.784, 115.582, 63.538 |
| Unit cell angles | 90.00, 101.06, 90.00 |
Refinement procedure
| Resolution | 42.423 - 2.820 |
| Rwork | 0.211 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.405 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.423 | 2.921 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.313 | 1.486 |
| Number of reflections | 12448 | 1221 |
| <I/σ(I)> | 6.84 | 1.75 |
| Completeness [%] | 98.5 | 98.31 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.984 | 0.513 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | sodium formate, PEG3350, L-glutamine |
