8RP6

Aminodeoxychorismate synthase complex from Escherichia coli

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.968000
Spacegroup name	P 21 21 21
Unit cell lengths	79.505, 108.326, 179.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.434 - 2.450
Rwork	0.204
R-free	0.25370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.504
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.434	2.538
High resolution limit [Å]	2.450	2.450
Rmerge	0.146	1.699
Number of reflections	57850	5716
<I/σ(I)>	11.91	1.79
Completeness [%]	99.9	99.79
Redundancy	13.4	13.5
CC(1/2)	0.998	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	MES, magnesium sulfate heptahydrate, glycerol