8ROV
Human dectin-2 with dimerization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 74.211, 65.030, 81.582 |
| Unit cell angles | 90.00, 100.61, 90.00 |
Refinement procedure
| Resolution | 40.090 - 2.360 |
| R-factor | 0.2254 |
| Rwork | 0.222 |
| R-free | 0.28410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.887 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.710 | 2.450 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Number of reflections | 15183 | 1527 |
| <I/σ(I)> | 6.9 | 2 |
| Completeness [%] | 96.0 | 96.7 |
| Redundancy | 5.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 3.35K, 90 mM MES, pH 6.0, 200 mM CaCl2 |
