8RO8
Human cohesin SMC1A-HD(longCC-EQ)/RAD21-C complex - Apo closed P-loop conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.979950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.550, 67.069, 51.629 |
| Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
| Resolution | 44.062 - 1.902 |
| R-factor | 0.1971 |
| Rwork | 0.195 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.771 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.902 | 1.902 |
| Rmerge | 0.091 | 1.545 |
| Number of reflections | 42535 | 6755 |
| <I/σ(I)> | 9.56 | 1.02 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.998 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium malonate dibasic monohydrate; 0.1 M Bis-Tris propane pH 6.5; 20% w/v PEG 3350 |
