8RO7

Human cohesin SMC1A-HD(longCC)/RAD21-C complex - Open/closed P-loop conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978570
Spacegroup name	C 1 2 1
Unit cell lengths	187.600, 64.590, 47.130
Unit cell angles	90.00, 103.97, 90.00

Refinement procedure

Resolution	32.299 - 2.093
R-factor	0.2
Rwork	0.199
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.905
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.220
High resolution limit [Å]	2.093	2.093
Rmerge	0.060	1.206
Number of reflections	32126	4997
<I/σ(I)>	12.64	1.32
Completeness [%]	98.9	95.5
Redundancy	4.6	4.7
CC(1/2)	0.999	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Hepes pH 7.0; 10% w/v PEG 6000