8RO7
Human cohesin SMC1A-HD(longCC)/RAD21-C complex - Open/closed P-loop conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.600, 64.590, 47.130 |
| Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
| Resolution | 32.299 - 2.093 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.905 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.093 | 2.093 |
| Rmerge | 0.060 | 1.206 |
| Number of reflections | 32126 | 4997 |
| <I/σ(I)> | 12.64 | 1.32 |
| Completeness [%] | 98.9 | 95.5 |
| Redundancy | 4.6 | 4.7 |
| CC(1/2) | 0.999 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Hepes pH 7.0; 10% w/v PEG 6000 |
