8RNZ

Metal-binding domain 3 of copper-transporting ATPase RAN1 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-15
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.8856
Spacegroup name	P 21 2 21
Unit cell lengths	50.755, 51.481, 62.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.480 - 1.980
R-factor	0.1942
Rwork	0.192
R-free	0.23490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.625
Data reduction software	DIALS (3.17.0)
Data scaling software	DIALS (3.17.0)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21_5190)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.480	2.080
High resolution limit [Å]	1.980	1.980
Rmerge	0.120	0.558
Rmeas	0.131	0.604
Rpim	0.050	0.228
Number of reflections	22108	1670
<I/σ(I)>	5.81	0.74
Completeness [%]	99.8	99.24
Redundancy	6.7	7.1
CC(1/2)	0.996	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	285.15	precipitant: 200 mM sodium chloride, 100 mM MES pH 6.5, 10% (w/v) PEG 4000 protein solution: 4.8 mg/mL protein, 300 mM sodium chloride, 50 mM HEPES pH 7.2