8RNG

Crystal structure of HLA B*18:01 in complex with TEVETYVL, an 8-mer epitope from Influenza A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953732
Spacegroup name	P 21 21 21
Unit cell lengths	50.647, 81.144, 110.176
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.020 - 1.450
R-factor	0.1872
Rwork	0.186
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.249
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.020	1.470
High resolution limit [Å]	1.450	1.450
Number of reflections	81219	3941
<I/σ(I)>	14.9	3.5
Completeness [%]	100.0	100
Redundancy	9.1
CC(1/2)	0.998	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Potassium Nitrate, 20% w/v PEG 3350 at pH 6.5