8RMT

Galectin-3 with a bound inhibitor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	34.410, 57.800, 62.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.304 - 1.250
Rwork	0.164
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.705
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.319	1.220
High resolution limit [Å]	1.150	1.150
Rmeas	0.111	2.790
Number of reflections	44848	7109
<I/σ(I)>	9.83
Completeness [%]	99.8	99.5
Redundancy	6.3	6.09
CC(1/2)	0.999	0.207

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.4 M Sodium malonate pH 7.0 (Index/C3)