Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 93.515, 93.515, 177.817 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.990 - 2.480 |
| R-factor | 0.22119 |
| Rwork | 0.219 |
| R-free | 0.26818 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.985 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.990 | 2.850 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.148 | 1.546 |
| Rmeas | 0.153 | 1.598 |
| Rpim | 0.040 | 0.399 |
| Number of reflections | 16422 | 820 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 93.6 | |
| Redundancy | 14.9 | |
| CC(1/2) | 0.999 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.1 M Ammonium Sulphate 0.1 M HEPES pH 7.5 0.7% n-octyl-beta-d-glucoside |
