Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.027, 94.027, 178.065 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.430 - 2.220 |
| R-factor | 0.22383 |
| Rwork | 0.222 |
| R-free | 0.25755 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.377 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.430 | 2.387 |
| High resolution limit [Å] | 2.220 | 2.221 |
| Rmerge | 0.107 | 1.964 |
| Rmeas | 0.110 | 2.038 |
| Rpim | 0.028 | 0.539 |
| Number of reflections | 37981 | 1900 |
| <I/σ(I)> | 16.1 | 14.4 |
| Completeness [%] | 94.8 | 59.2 |
| Redundancy | 94.8 | |
| CC(1/2) | 0.998 | 0.525 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.1 M Ammonium sulphate 0.1 M HEPES pH 7.5 0.7% n-octyl-beta-d-glucoside |
