8RLX
Erk2 MAP kinase T188A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-04 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.965 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.566, 69.510, 59.422 |
| Unit cell angles | 90.00, 109.17, 90.00 |
Refinement procedure
| Resolution | 43.670 - 1.600 |
| R-factor | 0.21172 |
| Rwork | 0.210 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.739 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.820 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 49171 | 2442 |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 99.3 | |
| Redundancy | 2.7 | |
| CC(1/2) | 0.993 | 0.881 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M sodium hepes pH=7, 4% v/v PEG 400 and 2M ammonium sulfate |
