8RLI

Neutron structure of perdeuterated hen egg-white lysozyme at room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	NUCLEAR REACTOR
Source details	ILL BEAMLINE D19
Synchrotron site	ILL
Beamline	D19
Temperature [K]	298
Detector technology	POSITION SENSITIVE DETECTOR
Collection date	2020-02-12
Detector	5ATM 3HE, CURVED 120X30 DEGREES
Wavelength(s)	1.455
Spacegroup name	P 1
Unit cell lengths	27.120, 31.630, 34.150
Unit cell angles	89.16, 72.05, 67.74

Refinement procedure

Resolution	9.960 - 0.910
Rwork	0.198
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.960	0.940
High resolution limit [Å]	0.910	0.910
Rmerge	0.076	1.120
Rmeas	0.089	1.451
Rpim	0.045	0.908
Number of reflections	67975	6381
<I/σ(I)>	5.5	0.7
Completeness [%]	95.5	89.1
Redundancy	2.3	1.7
CC(1/2)	0.997	0.148

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.5	291	0.15 M sodium nitrate, 0.05 M sodium acetate pH 4.5