8RLH

Neutron structure of hydrogenated hen egg-white lysozyme at room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	NUCLEAR REACTOR
Source details	ILL BEAMLINE D19
Synchrotron site	ILL
Beamline	D19
Temperature [K]	298
Detector technology	POSITION SENSITIVE DETECTOR
Collection date	2020-09-10
Detector	5ATM 3HE, CURVED 120X30 DEGREES
Wavelength(s)	1.458
Spacegroup name	P 1
Unit cell lengths	27.290, 31.940, 34.350
Unit cell angles	88.64, 71.51, 68.17

Refinement procedure

Resolution	9.960 - 1.100
Rwork	0.186
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.960	1.140
High resolution limit [Å]	1.100	1.100
Rmerge	0.089	0.585
Rmeas	0.107	0.769
Rpim	0.056	0.494
Number of reflections	38932	3703
<I/σ(I)>	5.7	1.5
Completeness [%]	94.5	88.8
Redundancy	2.2	1.6
CC(1/2)	0.993	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4.5	291	2 % NaNO3, 0.1 M Na acetate pH 4.5