8RLF
Hydrogenated hen egg-white lysozyme at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2016-09-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.688 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.250, 31.930, 34.270 |
| Unit cell angles | 88.52, 71.37, 68.16 |
Refinement procedure
| Resolution | 21.670 - 0.960 |
| Rwork | 0.097 |
| R-free | 0.12360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.670 | 0.990 |
| High resolution limit [Å] | 0.960 | 0.960 |
| Rmerge | 0.121 | 0.481 |
| Rmeas | 0.131 | 0.530 |
| Rpim | 0.050 | 0.221 |
| Number of reflections | 61660 | 6128 |
| <I/σ(I)> | 16.5 | 4.7 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 6.6 | 5.7 |
| CC(1/2) | 0.963 | 0.866 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4.5 | 291 | 2 % NaNO3, 0.1 M Na acetate pH 4.5 |
