8RKZ

Crystal structure of a cysteine hydrolase from Phytophthora infestans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-21
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	81.340, 218.350, 101.590
Unit cell angles	90.00, 102.41, 90.00

Refinement procedure

Resolution	47.830 - 2.300
R-factor	0.193
Rwork	0.191
R-free	0.23470
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.645
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.830	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.191	1.169
Number of reflections	152006	15213
<I/σ(I)>	6.91	1.13
Completeness [%]	99.4
Redundancy	3.8
CC(1/2)	0.991	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M di-Ammonium tartrate; 20 % (w/v) PEG 3350