8RKI
Molecular basis of ZP3/ZP1 heteropolymerization: crystal structure of a native vertebrate egg coat filament fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.07227 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 108.350, 108.350, 255.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 88.060 - 4.200 |
R-factor | 0.3305 |
Rwork | 0.329 |
R-free | 0.36770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold2 models |
RMSD bond length | 0.003 |
RMSD bond angle | 0.629 |
Data reduction software | XDS (Feb 5, 2021 BUILT=20210323) |
Data scaling software | XSCALE (Feb 5, 2021 BUILT=20210323) |
Phasing software | PHASER (2.8.3) |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.060 | 4.524 |
High resolution limit [Å] | 4.200 | 4.200 |
Rmerge | 0.207 | 2.457 |
Rmeas | 0.224 | 2.649 |
Rpim | 0.085 | 0.965 |
Number of reflections | 13155 | 2583 |
<I/σ(I)> | 4.42 | 0.81 |
Completeness [%] | 98.5 | 99.2 |
Redundancy | 6.4 | 6.8 |
CC(1/2) | 0.994 | 0.712 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 5% (w/v) PEG 20000, 25% (w/v) trimethylpropane, 0.1 M N,N-bis(2-hydroxyethyl)-2-amino ethanesulfonic acid (BES)/triethanolamine pH 7.5, 1% (w/v) non-detergent sulfobetaine (NDSB) 195, 0.5 mM YCl3, 0.5 mM ErCl3, 0.5 mM TbCl3, 0.5 mM YbCl3 |