8RJ8

CytK nanopore mutant

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.8856
Spacegroup name	P 1 21 1
Unit cell lengths	103.229, 186.222, 104.532
Unit cell angles	90.00, 115.85, 90.00

Refinement procedure

Resolution	94.070 - 4.320
Rwork	0.322
R-free	0.40700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.973
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.070	4.470
High resolution limit [Å]	4.320	4.320
Number of reflections	23236	1713
<I/σ(I)>	9.2
Completeness [%]	95.7
Redundancy	8
CC(1/2)	0.900	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.005 M Manganese(II) chloride tetrahydrate, 0.005 M Cobalt(II) chloride hexahydrate, 0.005 M Nickel(II) chloride hexahydrate, 0.005 M Zinc acetate dihydrate, 0.1 M MOPSO, Bis-Tris, pH 6.5, 35 %(w/v) glycerol, 30 %(w/v) PEG 4000