Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.687, 156.104, 182.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.990 - 2.570 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.22820 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.450 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21rc1_5058) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.990 | 2.600 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.103 | 3.169 |
| Rmeas | 0.111 | 3.406 |
| Rpim | 0.041 | 1.242 |
| Number of reflections | 181996 | 6020 |
| <I/σ(I)> | 10.58 | 0.51 |
| Completeness [%] | 99.3 | |
| Redundancy | 7.3 | 7.3 |
| CC(1/2) | 0.999 | 0.175 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 4% PEG 4K, 9-10% glycerol, 30 mM MgCl2, 30 mM CaCl2, 5 mM tyrosine and 100 mM MES/Imidazole pH 6.5 |
