8RIM

Arginase 2 in complex with inhibitor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-22
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	48.140, 134.550, 145.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.110 - 1.900
R-factor	0.25
Rwork	0.248
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.090
Data scaling software	Aimless
Refinement software	BUSTER (2.11.7 PACIOREK)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.360	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.056	0.849
Rmeas	0.062	0.933
Rpim	0.026	0.383
Total number of observations	419366	30662
Number of reflections	74804	5348
<I/σ(I)>	12.9	1.4
Completeness [%]	99.8
Redundancy	5.6	5.7
CC(1/2)	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	13-18% PEG3350, 0.2-0.35M Am. Cit. pH7.0