8RID

Crystallographic structure of oligosaccharide dehydrogenase from Pycnoporus cinnabarinus bound to Sinapic Acid, orthorhombic crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 21 21 21
Unit cell lengths	49.060, 61.883, 195.597
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.990 - 1.300
R-factor	0.1443
Rwork	0.143
R-free	0.17824
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.746
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.990	1.297
High resolution limit [Å]	1.190	1.190
Rmerge	0.076	1.119
Number of reflections	129361	6468
<I/σ(I)>	14	1.7
Completeness [%]	95.6
Redundancy	12.8
CC(1/2)	0.999	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	2 M AMMONIUM SULFATE