8RGI

Structure of DYNLT1:DYNLT2B (TCTEX1:TCTEX1D2) heterodimer.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-08
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.97856
Spacegroup name	P 41 21 2
Unit cell lengths	78.149, 78.149, 112.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.680 - 2.020
R-free	0.25860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.18_3845:)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.680	2.120
High resolution limit [Å]	2.020	2.020
Number of reflections	24180	3754
<I/σ(I)>	14.56
Completeness [%]	99.5
Redundancy	6.08
CC(1/2)	0.990	0.265

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	DYNLT1-DYNLT2B was crystallized using the hanging-drop vapour diffusion method by mixing 1 ul of protein solution (15 mg/ml) with 1-2 ul of precipitant (1.2-1.4 M potassium sodium tartrate, Tris pH 8-8.5) and incubating at 20 degrees Celsius. Crystals were cryo-protected in mother liquor supplemented with 25-30% glycerol and then flash frozen in liquid nitrogen.