8RG1
Soluble glucose dehydrogenase from acinetobacter calcoaceticus - wild type pH8
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM07 |
| Synchrotron site | ESRF |
| Beamline | BM07 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.354, 92.508, 84.711 |
| Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
| Resolution | 42.560 - 1.190 |
| R-factor | 0.1484 |
| Rwork | 0.148 |
| R-free | 0.17280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.083 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.233 |
| High resolution limit [Å] | 1.180 | 1.190 |
| Rmerge | 0.420 | |
| Rmeas | 0.590 | |
| Rpim | 0.420 | |
| Number of reflections | 199471 | 1815 |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 98.7 | 6.35 |
| Redundancy | 6.7 | 2 |
| CC(1/2) | 0.999 | 0.648 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 6000, 100 mM TRIS pH8, 2 mM ZnCl2 |
