8RFK
Soluble glucose dehydrogenase from acinetobacter calcoaceticus - single mutant pH8
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.733, 92.614, 85.341 |
| Unit cell angles | 90.00, 105.24, 90.00 |
Refinement procedure
| Resolution | 42.730 - 1.560 |
| R-factor | 0.1589 |
| Rwork | 0.158 |
| R-free | 0.18540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.799 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 1.630 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 1.000 | |
| Rmeas | 0.132 | 1.590 |
| Number of reflections | 131392 | 18670 |
| <I/σ(I)> | 10.7 | 0.58 |
| Completeness [%] | 97.7 | 86 |
| Redundancy | 6.4 | |
| CC(1/2) | 0.998 | 0.360 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | PEG 6000, TRIS, Lithium chloride |
