8RF7
Crystal structure of maize adenosine kinase 2 (ADK2) apoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 136.222, 136.222, 78.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.070 - 2.051 |
| R-factor | 0.2352 |
| Rwork | 0.234 |
| R-free | 0.26150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.324 | 2.180 |
| High resolution limit [Å] | 2.051 | 2.051 |
| Rmerge | 2.875 | |
| Number of reflections | 30369 | 1520 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 65.2 | |
| Redundancy | 26.4 | |
| CC(1/2) | 0.998 | 0.405 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Ammonium sulfate, sodium acetate, sodium fluoride |
