8REG

Lysozyme measured via serial crystallography from a kapton HARE-chip (50 micron)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2023-05-04
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.976
Spacegroup name	P 43 21 2
Unit cell lengths	79.000, 79.000, 38.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.860 - 1.700
R-factor	0.1632
Rwork	0.163
R-free	0.16850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.893
Data reduction software	CrystFEL (0.10.2)
Data scaling software	CrystFEL (0.10.2)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.860	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	15684	1349
<I/σ(I)>	12.54	8.68
Completeness [%]	100.0	100
Redundancy	571.5
CC(1/2)	0.988	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293.15	100mg/ml NaCl