8RE2
Crystal Structure determination of Dye-decolorizing Peroxidase (DyP) from Deinoccoccus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 32 |
| Unit cell lengths | 64.130, 64.130, 111.322 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.310 - 2.200 |
| R-factor | 0.1875 |
| Rwork | 0.183 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.963 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MoRDa |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.540 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rpim | 0.035 | 0.347 |
| Number of reflections | 25843 | 4160 |
| <I/σ(I)> | 10.96 | |
| Completeness [%] | 99.5 | |
| Redundancy | 4.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293.15 | 18% PEG 8000, 0.1 M CHES pH 8.5 |
