8RE0
Soluble glucose dehydrogenase from acinetobacter calcoaceticus - double mutant pH8
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.733, 92.614, 85.341 |
| Unit cell angles | 90.00, 105.24, 90.00 |
Refinement procedure
| Resolution | 42.730 - 1.560 |
| R-factor | 0.1613 |
| Rwork | 0.160 |
| R-free | 0.18350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.962 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.622 |
| High resolution limit [Å] | 1.560 | 1.566 |
| Rmerge | 0.055 | 0.467 |
| Rmeas | 0.077 | 0.659 |
| Number of reflections | 104245 | 1089 |
| <I/σ(I)> | 12.89 | 1.37 |
| Completeness [%] | 81.5 | 8.5 |
| Redundancy | 2 | |
| CC(1/2) | 0.995 | 0.468 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% PEG 6000, 100mM TRIS pH8, 200 mM LiCl2 |
