8RDR

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with spiro-isoxazol based compound 8g

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-11
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	56.663, 60.446, 88.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.820 - 1.580
R-factor	0.18427
Rwork	0.183
R-free	0.21430
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.621
Data reduction software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.300	49.300	1.680
High resolution limit [Å]	1.580	4.720	1.580
Rmeas	0.102	0.053	1.482
Number of reflections	80830	3027	13073
<I/σ(I)>	10	32.54	1.1
Completeness [%]	100.0	99.8	99.8
Redundancy	6.87	6.51	6.85
CC(1/2)	0.998	0.998	0.567

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3 mM thalidomide