8RDQ

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with spiro-isoxazol based compound 8b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-11
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.0001
Spacegroup name	P 21 21 21
Unit cell lengths	56.536, 59.800, 88.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.610 - 2.030
R-factor	0.20724
Rwork	0.205
R-free	0.24929
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.494
Data reduction software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.610	47.610	2.150
High resolution limit [Å]	2.030	6.040	2.030
Rmeas	0.163	0.055	1.849
Number of reflections	263076	1402	6032
<I/σ(I)>	8.86	32.77	1.03
Completeness [%]	99.9	99.6	99.7
Redundancy	7.06	7.3	7.24
CC(1/2)	0.997	0.998	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3 mM thalidomide