8RDC
Galectin-1 in complex with thiogalactoside derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.995, 70.035, 99.508 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.820 - 1.700 |
| R-factor | 0.17378 |
| Rwork | 0.172 |
| R-free | 0.20002 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.423 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.990 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 268479 | 13804 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.999 | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 30% PEG 3350, 0.1 M Tris/HCl pH 8.0, 0.2 M Li2SO4, 1 % beta mercapthoethanol, seeded |
