8RCI

Human p53 DNA-binding domain bound to DARPin C10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	37.692, 93.897, 53.168
Unit cell angles	90.00, 110.17, 90.00

Refinement procedure

Resolution	46.900 - 1.500
R-factor	0.153439775813
Rwork	0.151
R-free	0.19676
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.766
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.950	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.062	0.837
Rpim	0.029	0.387
Number of reflections	54680	2696
<I/σ(I)>	13.8	2
Completeness [%]	98.6
Redundancy	5.7
CC(1/2)	0.998	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Protein solution: 2.5 mg/ml of p53 DBD-DARPin C10 complex (1:1 ratio) in 50 mM Tris, pH 8, 150 mM NaCl, 0.5 mM TCEP Crystallization buffer: 25 % w/v PEG 3350