8RCA
W-formate dehydrogenase from Desulfovibrio vulgaris - Co-crystallized with Formate and Reoxidized by exposure to air for 1 h in the absence of Formate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-03 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.7749 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.558, 128.006, 148.905 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.258 - 1.664 |
| Rwork | 0.194 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sdv |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.258 | 1.792 |
| High resolution limit [Å] | 1.664 | 1.664 |
| Number of reflections | 108396 | 5420 |
| <I/σ(I)> | 7.992 | |
| Completeness [%] | 90.6 | |
| Redundancy | 4.66 | |
| CC(1/2) | 0.996 | 0.628 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 22% PEG 3350, 0.1M Tris-HCl pH 8.0, 1M LiCl |
