8RBJ

Structure of fungal tRNA ligase in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-12
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8731
Spacegroup name	P 21 21 21
Unit cell lengths	51.560, 75.360, 124.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.640 - 2.370
R-factor	0.1803
Rwork	0.178
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.939
Data reduction software	XDS (20200131)
Data scaling software	Aimless (0.7.12)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.640	2.460
High resolution limit [Å]	2.370	2.370
Rmerge	0.355	3.123
Rpim	0.090	0.856
Number of reflections	20384	2019
<I/σ(I)>	7.7	1
Completeness [%]	100.0
Redundancy	15.8
CC(1/2)	0.989	0.392

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	protein concentration: 5.8 mg/ml protein:RNA ratio = 1:1.7 precipitant solution: 20 % PEG3350, 0.2 M potassium formate