8RBI

Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant H124

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.6525
Spacegroup name	C 1 2 1
Unit cell lengths	76.520, 38.450, 74.010
Unit cell angles	90.00, 116.62, 90.00

Refinement procedure

Resolution	33.520 - 0.980
R-factor	0.1239
Rwork	0.124
R-free	0.14860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.150
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_4788)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.520	1.000
High resolution limit [Å]	0.980	0.980
Number of reflections	213697	6866
<I/σ(I)>	17.36
Completeness [%]	98.6
Redundancy	6.8
CC(1/2)	1.000	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	30% PEG3350, 0.1M Bis-Tris pH 5.5, 0.2M NaCl