8RBH
Crystal structure of the kelch domain of human KLHL12 in complex with PEF1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-08 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976254 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.001, 78.390, 73.784 |
| Unit cell angles | 90.00, 99.24, 90.00 |
Refinement procedure
| Resolution | 72.830 - 1.880 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.830 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.283 | 3.843 |
| Rmeas | 0.306 | 4.154 |
| Rpim | 0.115 | 1.562 |
| Number of reflections | 40172 | 1936 |
| <I/σ(I)> | 6.2 | 0.6 |
| Completeness [%] | 97.6 | 94.58 |
| Redundancy | 6.928 | |
| CC(1/2) | 0.992 | 0.189 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 0.1 M BIS-TRIS pH 5.5, 200 mM NaCl, and 30% PEG4K. (1:1) |
