8RBE
Crystal structure of Mycobacterium tuberculosis MmaA1 in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.616, 74.840, 97.595 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 19.790 - 1.900 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.20220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.617 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.125 | 1.198 |
| Rmeas | 0.140 | 1.344 |
| Rpim | 0.063 | 0.599 |
| Number of reflections | 47320 | 4673 |
| <I/σ(I)> | 9.13 | 1.16 |
| Completeness [%] | 99.8 | 99.83 |
| Redundancy | 4.6 | 4.7 |
| CC(1/2) | 0.997 | 0.533 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M HEPES (pH 7.5), 10% (v/v) 2-propanol 20% (w/v) polyethylene glycol 4,000 |
