8RAY
ParA in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.885600 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.740, 85.780, 97.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.720 - 1.580 |
| R-factor | 0.1749 |
| Rwork | 0.174 |
| R-free | 0.19980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.591 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.720 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.136 | 2.179 |
| Number of reflections | 67745 | 5683 |
| <I/σ(I)> | 12.99 | |
| Completeness [%] | 98.3 | |
| Redundancy | 13.2 | |
| CC(1/2) | 0.999 | 0.673 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 10% glycerol, 0.1M MES, 30% PEG-600, 5% PEG-1000 |
