8R8C

Structure of the N-terminal domain of CMA from Cucumis melo in complex with N-acetylgalactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	I 1 2 1
Unit cell lengths	36.613, 36.860, 94.813
Unit cell angles	90.00, 99.17, 90.00

Refinement procedure

Resolution	35.680 - 1.550
Rwork	0.163
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.893
Data reduction software	XDS
Data scaling software	Aimless (0.7.13)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.680	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.052	0.496
Rmeas	0.064	0.618
Rpim	0.037	0.364
Number of reflections	18279	4581
<I/σ(I)>	14.4	2.9
Completeness [%]	99.7
Redundancy	5.2
CC(1/2)	0.998	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	12% Peg Smear Medium 0.1M Mes pH 6.5 5 mM of CaCl2, MgCl2, CsCl2, CdCl2, NiCl2 and Zinc acetate and GalNAc