8R8B
Wnt binding to COPalpha and COPBeta2 directs secretion on extracellular vesicles
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979335 |
| Spacegroup name | P 1 |
| Unit cell lengths | 160.457, 160.656, 160.619 |
| Unit cell angles | 107.27, 107.16, 107.24 |
Refinement procedure
| Resolution | 49.377 - 1.808 |
| Rwork | 0.159 |
| R-free | 0.17440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ynn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 139.200 | 1.920 |
| High resolution limit [Å] | 1.808 | 1.810 |
| Number of reflections | 1088963 | 178778 |
| <I/σ(I)> | 8.53 | |
| Completeness [%] | 95.5 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.990 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 0.1M Hepes 7.0 2.0M AmSO4 |
