8R7I
X-ray structure of blue laccase BP76 from the termite Neocapritermes taracua
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.362, 0.918 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 163.500, 163.500, 89.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.200 - 1.800 |
| R-factor | 0.13253 |
| Rwork | 0.132 |
| R-free | 0.14484 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.585 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.200 | 47.200 | 1.910 |
| High resolution limit [Å] | 1.800 | 5.360 | 1.800 |
| Rmerge | 0.135 | 0.053 | 0.821 |
| Rmeas | 0.143 | 0.056 | 0.864 |
| Number of reflections | 126421 | 5011 | 20205 |
| <I/σ(I)> | 12.12 | ||
| Completeness [%] | 99.7 | ||
| Redundancy | 10 | ||
| CC(1/2) | 0.998 | 0.999 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 291 | 100 mM sodium acetate pH 4.5, 2 M ammonium sulfate |
