8R7D

Complex of rice blast (Magnaporthe oryzae) mutant effector protein PWL2-SNDE with the HMA domain of OsHIPP43 from rice (Oryza sativa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	66.378, 66.804, 81.901
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.130 - 2.500
R-factor	0.21971
Rwork	0.216
R-free	0.28582
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.725
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.130	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.285	1.351
Rmeas	0.296	1.402
Rpim	0.080	0.374
Total number of observations		20237
Number of reflections	13157	1454
<I/σ(I)>	6.6	1.9
Completeness [%]	100.0
Redundancy	13.3	13.9
CC(1/2)	0.992	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Ammonium formate, 20% PEG 3350