8R7A

Complex of rice blast (Magnaporthe oryzae) effector protein PWL2 with the HMA domain of OsHIPP43 from rice (Oryza sativa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 61 2 2
Unit cell lengths	63.161, 63.161, 198.208
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.760 - 1.800
R-factor	0.1897
Rwork	0.188
R-free	0.21510
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.619
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.760	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.086	1.676
Rmeas	0.087	1.698
Rpim	0.014	0.271
Total number of observations		49513
Number of reflections	22749	1291
<I/σ(I)>	31.1	2.9
Completeness [%]	100.0
Redundancy	36.9	38.4
CC(1/2)	1.000	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris 8.0