8R7A
Complex of rice blast (Magnaporthe oryzae) effector protein PWL2 with the HMA domain of OsHIPP43 from rice (Oryza sativa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-25 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.161, 63.161, 198.208 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.760 - 1.800 |
| R-factor | 0.1897 |
| Rwork | 0.188 |
| R-free | 0.21510 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.619 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.760 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.086 | 1.676 |
| Rmeas | 0.087 | 1.698 |
| Rpim | 0.014 | 0.271 |
| Total number of observations | 49513 | |
| Number of reflections | 22749 | 1291 |
| <I/σ(I)> | 31.1 | 2.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 36.9 | 38.4 |
| CC(1/2) | 1.000 | 0.915 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Tris 8.0 |
