8R75

Polysaccharide lyase BtPL33HA (BT4410) Apo form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.6533
Spacegroup name	P 2 21 21
Unit cell lengths	74.370, 101.580, 161.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.506 - 2.700
Rwork	0.180
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	2.100
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.610	2.830
High resolution limit [Å]	2.700	2.700
Number of reflections	34414	4486
<I/σ(I)>	15.8	1.3
Completeness [%]	100.0
Redundancy	31.4
CC(1/2)	0.999	0.735

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	20 % PEG 3350 0.2 M Na/K Tartrate 20 mg/ml protein