8R73

Polysaccharide lyase BcPL33HA (BcellWH2_04512) Apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	103.630, 91.390, 84.770
Unit cell angles	90.00, 109.28, 90.00

Refinement procedure

Resolution	40.008 - 2.110
Rwork	0.189
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.826
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.010	2.170
High resolution limit [Å]	2.110	2.110
Number of reflections	42837	3507
<I/σ(I)>	10.8	1.5
Completeness [%]	99.7
Redundancy	6.7
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	10 % PEG 8000 8 % Ethylene glycol 0.1 M HEPES pH 7.5 28 mg/ml